Callus cultures of Thymus vulgaris and Trifolium pratense as a source of geroprotectors

Introduction. Geroprotectors are biologically active substances that inhibit the aging process. Many plant species natural geroprotectors. For instance, Thymus vulgaris and Trifolium pratense callus cultures with strong geroprotective properties.
 Study objects methods. The present research featured T. grown in vitro on various nutrient media. Their extracts were obtained by aqueous-alcoholic extraction using following parameters: water-ethanol solvent Se = 30, 50, 70 %; temperature Te 70°C; time τe 2, 4, 6 h. quantitative qualitative analysis was based high-performance liquid mass spectrometry, gas thin-layer chromatography.
 Results discussion. optimal parameters for 4 h, 50°C, %, – 70°C, %. chromatography detected flavonoids, phenylpropanoids, simple phenols, higher fatty acids, mono- sesquiterpenes, aliphatic hydrocarbons. appeared to have highest content of thymol (23.580 ± 1.170 mg/mL); its thymol, apigenin, gallic, chlorogenic, caffeic components demonstrated properties. extract possessed rutin (10.05 0.35 mg/mL), it owed characteristics rutin, chlorogenic p-coumaric acids.
 Conclusion. proved be promising sources substances.